Fixed HeLa cells that have internalised 15nm AuNP via Tf-mediated endocytosis imaged by (a) Differential interference contrast (DIC) microscopy (b) co-circularly polarised reflection amplitude A+2r (c) co-circularly polarised FWM amplitude A+FWM (d) confocal fluorescence microscopy. FWM was acquired with pump-probe delay time of 0.5ps, pump (probe) power at the sample of 50μW (25μW). Confocal fluorescence and FWM are shown as maximum intensity projection of z stacks which were acquired over 6μm in 300nm z-steps, while the reflection is on a single xy plane.

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